Sophisticated Analytical Instrumentation Facility, CIL and UCIM

High Resolution Transmission Electron Microscope

 

SPECIFICATIONS:

• Working Voltage : 80 to 200 kV,
• Magnification : 15,000,00 lakh(Max),
• Resolution : 0.194 nm (point), Lattice: 0.14 nm
• Detector: Charge Couple Device (CCD)

SAMPLE REQUIREMENTS:

• If you want to stain your sample (positive or negative stain), please bring prepared stain at appropriate pH and concentration with proper filtration.
• Submit your sample in powder form. If it is already dispersed in solvent, it should not be other than water/ethanol.
• Samples must be prepared/submitted at least one-day advance.
• Magnetic samples/unstable samples under vacuum are not acceptable for analysis in HR-TEM.
• Sample should be dispersed after optimum sonication time as per the size range and requirement of the sample.
• If your sample is biological/life science sample (tissue, bacteria, plant sample etc.), please contact personally to HR-TEM lab.

Important thing must read before sending samples:

• If you want to stain your sample (positive or negative stain), please bring prepared stain at appropriate pH and concentration with proper filtration.
• Sample prepared by green synthesis must be washed (Centrifuged) & sonicated at appropriate conditions.
• If your sample size is below 10 nm, it should bring in pellet form to lab after proper centrifugation and sonication.
• Presence of user during imaging is appreciable.
• If sample received by post for HR-TEM analysis, results will be acceptable to user irrespective information provided.
• It is duty of user to ensure the quality and specific parameters of sample related to TEM Analysis.
• Samples must be prepared/submitted at least one day advance.

Instruction for HRTEM:
Per sample processing time is 1 hour maximum (Including sample loading & unloading ).
If you need extra analysis on same sample, the instrument time will be charged as one/more sample(s).

Instruction for EDS and mapping analysis:
Please specify the probable elements which can be detected EDS/Mapping mode.

Note:
Microscopy will be done only if the sample has been characterized earlier by other techniques such as XRD, SEM, XPS, TG etc. 200kV UHRTEM instrument is not a test equipment for preliminary investigation of sample. Please ensure this before bringing sample to lab.

HRTEM (Per Sample)	SAED (Per Sample)	TEM- EDS /Mapping (per Sample)	STEM-BF/DF (per Sample)
Max.5 Images will be given per sample.Rs.50 per image will be charged for extra image.( Max 3 images given)	Single Diffraction Pattern	Single spectrum/Mapping	4 images = 2 Bright Field + 2 Dark Field

If user want any mode analysis namely SAED,MAPPING/EDS/STEM-BF/DF they have to do TEM analysis also.

Contact Details:
Landline for appointment: +91 172 2534048, 2541065.
Email id : temlab@pu.ac.in